This page explains how to upload datasets and generate reports.

Click New Report in the top menu bar. This will take you to the New Report page.

The page is divided into three sections. Work through them top to bottom.

1) Enter report details

The first section covers general aspects of the experiment that determine how your data will be processed.

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• Report name: Title that will appear at the top of the report. You can change this later.
• Project: Project report falls under. This also determines what users can see your report. See section on Managing users.
• Load FCS from previous report: Reuse settings from previous upload [COMING SOON].
• Data type: Flow cytometry or CyTOF.
• Cell type: Pregated cell type. For example, if you pregated CD3+ T cells, choose T cells. If your dataset contains multiple cell types or your cell type is not listed, choose Unspecified.
• Topics of interest: Research topics used to refine literature results. Topics are based on MeSH terms and can include diseases, molecules, and immune processes. This field is optional and can be added or changed later.

2) Upload FCS files

Scroll down to the Samples section. Use this section to upload FCS files and assign them to keyword groups. These groups set up the statistical comparisons performed later.

Upload files

Click Upload Folder to upload an entire folder or Upload Files to select individual files. Navigate to your files and click Open. Repeat the process to add more files.

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<aside> 🛁

Do I have to clean up my files?

Upload clean, compensated FCS files (e.g., live, singlet, CD3+ T cells). Clean up files in your preferred gating software and export the target population for downstream analysis. You do not need to rescale your data.

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Review keywords

terraFlow automatically assigns sample keywords based on file names and folder structure. Scan the table to make sure keyword assignments are correct.